A Dextran Test for Vhp Sugars
نویسنده
چکیده
The monoclonal antibody (MCA) SucroTest is a commercially available kit (Midland Research Laboratories Inc) for the specific analysis of dextran in juices and sugar. The method is simple and quick and is based on the selective and specific reaction between the antibody and its antigen (in this case dextran). The resultant insoluble complex causes turbidity which is measured after a set time using a nephelometer and single point calibration against a commercial high molecular weight dextran standard. Very high pol (VHP) sugars from the South African Sugar Terminal (SAST) were analysed using the MCA-SucroTest. Repeatability was slightly worse than that of the haze method. The correlation between the MCA and haze method was good. Although the MCA results were, on average, 20% higher than the haze data, the ease and speed of the analysis lends itself to the application of a screening and rejection protocol for VHP sugars.
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